DNA / RNA / PCR Reagents
PCR amplification is an invaluable tool for analyzing genomic data from DNA samples. Various reagents are needed to purify the sample, produce the copies, and stabilize the new clones. BioWORLD carries all of the lysis buffers, Taq DNA polymerase, and reaction reagents necessary to accomplish PCR amplification. DNA/RNA isolation kits contain each component used in the attached easy-to-follow protocols.
Annealing Buffer
$83.33
Annealing Buffer is commonly implicated in use with oligonucleotides. This buffer is optimized for resuspending and annealing oligonucleotides. more info
Betaine 5M for PCR
$62.14
Betaine is a methyl donor that can improve cell growth and increase PCR yield. It especially amplifies GC-rich DNA sequences by preventing the... more info
bioPLUS PCR Enhancer
$75.89
bio PLUS PCR enhancer is an optimized PCR additive reagent that allows efficient amplification of difficult templates with high GC content or... more info
Carlson Lysis Buffer for CTAB DNA Extraction
$98.51
Carlson Lysis Buffer is used in DNA purification of plant, bacteria, and fungal sources. CTAB lyses cell membranes to make the DNA accessible for... more info
Cell Lysis Buffer I for ChIP
$76.03
50mM Tris-HCL, 1% SDS, 10mM EDTA, pH to 8.1. Prepared in DI water and filtered through 1 � filter. Shipped at room temperature, store at 2-8 C. more info
Cell Lysis Buffer II for ChIP
$76.03
10 mM Tris-HCL, pH to 8.1, 10 mM NaCl, 0.2% NP-40. Prepared in DI water and filtered through 0.22 � filter. Shipped at room temperature, store... more info
Cell Lysis Buffer III for ChIP
$76.03
5 mM PIPES, 85 mM KCl and 0.5% NP40, pH 8.0. Prepared in DI water and filtered through 0.22 � filter. Shipped at room temperature, store at 2-8... more info
Cell Lysis Buffer IV for ChIP
$76.03
50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 1mM PMSF, pH 7.5. Prepared in DI water and filtered through 0.22 �... more info
Cell Lysis Buffer V for ChIP
$76.03
20mM Tris-HCl, 85 mM KCl, 0.5% NP-40, pH 8.0. Prepared in DI water and filtered through 0.22 � filter. Shipped at room temperature, store at 2-8... more info
ChIP Dilution Buffer - I
$53.85
20 mM Tris-HCl, 1% TritonX-100, 2mM EDTA, 150mM NaCL, and 0.1% SDS, pH 8.1. Prepared in DI water and filtered through 0.22 � filter. Shipped at... more info
ChIP Dilution Buffer - II
$53.85
16.7 mM Tris-HCl, 1.1% Titron X100, 1.2 mM EDTA, 167 mM NaCl, 0.01% SDS, pH 8.1. Prepared in DI water and filtered throgh 0.22 � filter. Shipped... more info
ChIP Extraction Solution I
$53.85
1% SDS and 0.1 M NaHCO3. Prepared in DI water and filtered through 0.22 � filter. Shipped at room temperature, store at RT. more info
ChIP Extraction Solution II
$53.85
10 mM Tris-HCl, 1 mM EDTA and 1% SDS, pH 8.0. Prepared in DI water and filtered through 0.22 � filter. Shipped at room temperature, store at RT. more info
ChIP High Salt Immune Complex Wash Buffer
$53.85
20 mM Tris-HCl, 500 mM NaCl, 1% Triton X100, 0.1% SDS, 2mM EDTA, pH 8.1. Prepared in DI water and filtered through 0.22 � filter. more info
ChIP LiCl Immune Complex Wash Buffer - 1
$53.85
10 mM Tris-HCl, 250 mM LiCl, 1% Triton X-100, 1.2 mM EDTA, 1% Sodium deoxycholate, pH 8.1. Prepared in DI water and filtered through 0.22 µ... more info
ChIP LiCl Immune Complex Wash Buffer - 2
$53.85
10 mM Tris-HCl, 250 mM LiCl, 0.5% Igepal CA630, 1mM EDTA 0.5% Sodium deoxycholate, pH 8.1. Prepared in DI water and filtered through 0.22 �... more info
ChIP Sonication Buffer
$53.73
50 mM Hepes, 140 mM NaCl, 1% Triton X100, 0.1% SDS, 0.1% Sodium Deoxy cholate, pH 7.9. Prepared in DI water and filtered through 0.22 � filter.... more info
ChIP Swelling Buffer
$53.73
ChIP Swelling Buffer has common applications in chromatin immunoprecipitation protocol. This buffer is a hypertonic solution that causes the cell to... more info
ChIP Wash Buffer A
$54.22
50 mM Hepes, 500 mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1%, Sodium Deoxy cholate and 0.1% SDS, pH 7.9. Prepared in DI water and filtered through 0.22... more info
ChIP Wash Buffer B
$53.73
20 mM Tris-HCl, 250 mM LiCl, 0.5% Sodium Deoxycholate 0.5% NP-40, pH 8.0. Prepared in DI water and filtered through 0.22 � filter. Shipped at... more info
Denhardt's Solution 100X, Sterile
$60.16
Denhardt's Solution acts as a blocking reagent during hybridization to prevent probe binding to open membrane space and non-target nucleic acids.... more info
Denhardt's Solution 50X, Sterile
$89.68
1% Ficoll (Type 400), 1% Polyvinylpyrrolidone and 1% Bovine Serum Albumin (fraction v). Prepared in sterile DI water. Shipped cold on blue ice,store... more info
Dextran Sulfate 40%, Sterile
$211.53
Dextran sulfate 40% sterile solution is used for preparation of hybridization cocktails. bioWORLD's products are supplied for LABORATORY RESEARCH... more info
dGTP, 100mM
$131.34
PCR Grade, dGTP solution supplied as a 100mM trisodium salt solution 2'-Deoxyguanosine-5'-triphosphate Stability (-20°C): >1year Max... more info
DMS Buffer
$133.36
Dimethyl suberimidate•2HCl A longer spacer imidoester cross-linker Category: amine-reactive Reactive Groups: imidoester Reaticive Toward: amino... more info
DNA Fish Testes Denatured Solution
$94.54
Source: Fish Testes Biotechnology Grade 20 mg/ml Solution Appearance: Liquid more info
DNA Hybridization Buffer
$127.91
EKONO® Provides real cost savings without sacrificing performance or reliability. Effective for Southern, Northern, Southwestern, and... more info
dNTP Mixture 10mM Each
$99.25
This dNTP Mixture is used for routine PCR amplifications. It contains 10mM each of dATP, dGTP, dCTP, and dTTP. Each solution in this dNTP mixture is... more info
dNTP Mixture 2.5mM Each
$229.86
This dNTP Mixture is used for routine PCR amplifications. It contains 2.5mM each of dATP, dGTP, dCTP, and dTTP. Each solution in this dNTP mixture is... more info
dNTP Set, 100mM Each
$297.04
Deoxynucleotide triphosphate (dNTP) set The set is prepared using ultra pure dNTPs and high quality molecular grade water ensuring consistency and... more info
dNTP, 100mM
$295.69
dNTP mixture contains 1 mL, 25mM each of dCTP, dTTP, dGTP, and dATP prepared in DEPC treated PCR grade DI water. Shipped on Blue Ice, store at -20C. more info
dTTP 100mM
$252.76
PCR Grade, dTTP solution supplied as a 100mM trisodium salt solution. Specification Ultra Pure Grade Produced by enzymatic synthesis Purity:... more info
Duplex Buffer, Sterile
$57.07
Duplex buffer contains 100 mM potassium acetate, 30 mM HEPES and has a pH level of 7.5. It comes as a sterile solution in DEPC-treated water and is... more info
dUTP, 100mM
$137.45
PCR Grade, dUTP solution supplied as a 100mM solution. Specification Ultra Pure Grade Produced by enzymatic synthesis Purity: >99.0% more info
Firepol Thermostable TAQ Polymerase
$155.28
A thermostable DNA polymerase FirePol is similar to Taq DNA polymerase I (homology 98%). 3' to 5' exonuclease activity. Has the "extendase... more info
GBS/PCR Buffer 50X pH 8.8, Sterile
$80.09
GBS/PCR Buffer 50X pH 8.8, Sterile is commonly used in a combined enrichment/PCR method for detecting the presence of Group B streptococcus. This... more info
GTE 1X, Sterile
$42.39
Sterile, nuclease-free buffers and reagents have been prepared with the finest Biotechnology Grade chemicals in specially purified water treated to... more info
GTE Buffer, Sterile
$38.38
50mM Glucose, 25mM Tris, and 10 mM EDTA, pH 7.4. Prepared in DI water and sterilized. Shipped at RT, store at 2-8 C. bioWORLD's products are... more info
LiCl ChIP Wash Buffer
$157.98
For use in Chromatin Immunoprecipitation assays. LiCl ChIP Wash Buffer is supplied as 1X working solution. Contains: 0.25M LiCl, 1% NP40, 1%... more info
Mac Ewen Buffer, Sterile
$55.30
NaCl (125 mM), KCl (5 mM, CaCl2 (2 mM), MgCl2 (1 mM), HEPES (8 mM), 11 mM glucose, pH 7.4 � 0.2. Prepared in DI water and filtered through 0.22... more info
Magnesium Stock Solution, 2M, Sterile
$43.42
101.5g MgCl2 • 6H2O 123.3g MgSO4 • 7H2O Sterile bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be... more info
NucleasEliminator™
$30.55
DNase and RNase contamination ruins expensive samples in research laboratories everyday. NucleasEliminator™ can help protect the investment by... more info
PCR Buffer Set (10X)
$41.29
PCR Buffer Set is an optimized PCR buffer set. PCR Buffer Set is used for amplification of specific DNA fragments in conventional PCR assays ( i.e .,... more info
PCR Master Mix 2X
$270.94
2X PCR Master Mix Applications • Routine PCR amplification of templates • PCR for post reverse transcription step Reagents supplied: •... more info
Pfu Reaction Buffer 10X
$63.94
200 mM Tris-HCl (pH 8.8), 100 mM ammonium sulphate, 100 mM KCl, 1% (v/v) Triton X-100, 1 mg/ml BSA. Prepared in DI water and filtered through 0.22... more info
Pfu Reaction Buffer with MgSO4 10X
$63.94
200 mM Tris-HCl (pH 8.8), 100 mM ammonium sulphate, 100 mM KCl, 1% (v/v) Triton X-100, 1 mg/ml BSA and 20 mM magnesium sulphate. Prepared in DI water... more info
QBT Buffer for Plasmid DNA Isolation
$52.93
Product Description QBT buffer is a plasmid DNA purification buffer. It is composed of 750 mM NaCl, 50 mM MOPS, 15% isopropanol, 0.15% Triton X-100... more info
QC Buffer for Plasmid DNA Isolation
$39.37
Product Description QC buffer is a plasmid DNA purification buffer. It is composed of 1.0M NaCl, 50 mM MOPS, 15% isopropanol and has a pH value of... more info
QF Buffer for Plasmid DNA Isolation
$39.37
Product Description QF buffer is a plasmid DNA purification buffer. It is composed of 1.25 M NaCl, 50 mM Tris-Cl, 15% isopropanol and has a pH value... more info